Tetrandrine Inhibits the Wnt/β-Catenin Signalling Pathway and Alleviates Osteoarthritis: An In Vitro and In Vivo Study
Figure 2
Effects of Tet on the expression of matrix metalloproteinase-1 (MMP-1), MMP-3, MMP-13, tissue inhibitors of metalloproteinase-1 (TIMP-1), and β-catenin in chondrocytes. Cells were pretreated with Tet (0 mg/L, 5 mg/L, 10 mg/L, 20 mg/L) for one hour, followed by stimulation with interleukin-1β (IL-1β) (10 ng/mL) for 24 h. qRT-PCR was performed to determine the expression levels of MMPs in chondrocytes in (a). The protein concentrations of MMP-3, TIMP-1, and β-catenin were detected by Western blot analysis with the optimal concentration (b). The protein concentrations of MMP-3, MMP-13, and TIMP-1 were quantitated (c). Data are expressed as mean ± SD. * compared with cells stimulated with IL-1β alone.
