Tetrandrine Inhibits the Wnt/β-Catenin Signalling Pathway and Alleviates Osteoarthritis: An In Vitro and In Vivo Study

Table 1

Primers of targeted genes.

Gene	Genbank accession	Primer sequences(5′ to 3′)	Size (bp)	Annealing (°C)
Rabbit MMP-1	M25663	F: CAGGAGCCTTCCCAAGAGGAA	79	62
		R: CTTGTCTCTTGCATATCAGGATGATG
Rabbit MMP-3	NM_001082280	F: ACACCGGATCTGCCAAGAGA	89	63
		R: CTGGAGAACGTGAGTGGAGTCA
Rabbit MMP-13	NM_001082037	F: CAGATGGGCATATCCCTCTAAGAA	88	63
		R: CCATGACCAAATCTACAGTCCTCAC
Rabbit TIMP-1	AY829730	F: CAACTGCGGAACGGGCTCTTG	102	63
		R: CGGCAGCGTAGGTCTTGGTGAA
Rabbit 𝛽-catenin	DQ786777	F: CGTACGCACCATGCAGAACACAAA	154	63
		R: ATCCACTGGTGAACCGAGCATCTT
Rabbit 18S	EU236696	F: GACGGACCAGAGCGAAAGC	119	62
		R: CGCCAGTCGGCATCGTTTATG