Inclusion Complex of Zerumbone with Hydroxypropyl-β-Cyclodextrin Induces Apoptosis in Liver Hepatocellular HepG2 Cells via Caspase 8/BID Cleavage Switch and Modulating Bcl2/Bax Ratio

Table 2

Flow cytometric analysis of Annexin V in HepG2 cells which were treated with 11.43 𝜇g/mL ZER-HP𝛽CD inclusion complex for 6, 12, and 24 hours.

Cell condition	Percentage of cells (%)
	Control	6 h	12 h	24 h
Viable	93.90±1.20	89.70±1.50	88.80±3.20	79.30±5.80
Early apoptosis	4.20±0.27	9.00±0.31^∗	8.80±0.44^∗	15.20±0.40^∗
Late apoptosis	1.90±0.12	1.30±0.53	2.30±0.35	5.50±0.27^∗

Data are shown as mean ± SD (𝑛=3); *Indicates a significant difference compared with control (𝑃<0.05).