Subtoxic Levels of Apigenin Inhibit Expression and Secretion of VEGF by Uveal Melanoma Cells via Suppression of ERK1/2 and PI3K/Akt Pathways
Figure 3
Dose- and time-effects of apigenin on the secretion of VEGF by human uveal melanoma cells. Cells were plated into 24-well plates. In the dose-effect study, apigenin at 0, 1, 2 and 5 μM was added to the culture and incubated for 24 h, (a) SP6.5 cell line and (b) C918 cell line. In the time-effect study, apigenin at 5.0 μM was added and conditioned medium was collected 6, 12 and 24 h later, (c) SP6.5 and (d) C918. The amount of VEGF protein in the conditioned medium was determined by using the human VEGF Quantikine ELISA kit. VEGF levels in the conditioned culture medium were expressed as the percentages of the controls. Apigenin significantly reduced the secretion of VEGF by uveal melanoma cells in a dose- and time-dependent manner. Data are mean ± SD (). , versus control (cells cultured without apigenin).
