Integration of Different “-omics” Technologies Identifies Inhibition of the IGF1R-Akt-mTOR Signaling Cascade Involved in the Cytotoxic Effect of Shikonin against Leukemia Cells
Figure 4
Effect of shikonin on the PI3K-mTOR signaling cascade. (a) U937 cells were treated with 0.3 μM shikonin for 24 h and subsequently stained with phospho-specific antibodies against p-Akt and p-ribosomal protein S6. Shikonin treatment significantly decreased the amount of phosphorylated Akt (left shift), but no effect on the phosphorylation status of ribosomal protein S6 was detected. (b) Statistical quantification of p-Akt or p-ribosomal protein S6 negative cells after shikonin treatment. Data points represent mean ± SD of at least three independent experiments. (*Significantly different according to Student’s t-test, ).
