Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells
Figure 2
Sub-A induced mitotic catastrophe in A549 and NCI-H460 cells. (a) The formation of multinucleated giant cells in Sub-A-treated cells. (b) Aberrant segregation of chromosomes. (c) Accumulation of p-MPM2. (d) Abnormal mitosis. Cells were treated with 30 μM Sub-A for 24 h and then were stained by DAPI, tubulin antibodies, p-MPM2 antibodies, or p-histone3/Lamin B antibodies. The samples were analyzed by confocal microscopy and the data were representative of three independent experiments.
