Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells

Figure 3

Sub-A induced apoptosis in A549 and NCI-H460 cells. (a) The DNA fragmentation of A549 and NCI-H460 cells induced by Sub-A, as determined by electrophoresis assay at 48 h. Quantitative evaluations of TUNEL assay by (b) flow cytometry and (c) fluorescence microscopy. Each value was the mean ± SD of three determinations. The asterisk indicated a significant difference between Sub-A-treated cells and the controls (𝑃<0.05, by ANOVA with Student’s 𝑡-test post hoc).

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