Subamolide A Induces Mitotic Catastrophe Accompanied by Apoptosis in Human Lung Cancer Cells
Figure 7
The role of ATF3 and p53 on Sub-A-mediated mitotic catastrophe. The inhibition of ATF3 decreased (a) ATF3 mRNA and (b) p53 levels in Sub-A-treated cells. (c) Knockdown of ATF3 reduced the Sub-A-mediated mitotic catastrophe. (d) The inhibition of p53 decreased the Sub-A-mediated mitotic catastrophe. Cells were transfected control or ATF3 shRNA and then incubated with 30 μM Sub-A for specific times (3 h for p53 and 24 h for mitotic catastrophe). For (d), cells were transfected pCMV or pCMVp53mt135 plasmid, and then incubated with 30 μM Sub-A for 24 h. The induction of mitotic catastrophe was determined by counting multinuclear giant cells after DAPI staining. Each value was the mean ± SD of three determinations. The asterisk indicated a significant difference between Sub-A-treated cells and the controls (, by ANOVA with Student’s -test post hoc).
