Oral Administration of Alkylglycerols Differentially Modulates High-Fat Diet-Induced Obesity and Insulin Resistance in Mice

Table 3

The effect of selachyl alcohol (SA) or batyl alcohol (BA) supplementation on serum cytokines and leptin before and after 100 ng lipopolysaccharide (LPS) challenge.

		Pre-LPS^1
							P value^4	P value	P value
Item	ND^2	HF	HF + 20 mg/kg SA	HF + 200 mg/kg SA	HF + 20 mg/kg BA	HF + 200 mg/kg BA	(HF)	(SA)	(BA)
IL-1β (pg/mL)	2.3±0.19^3	8.8±0.70	8.25±1.09	7.1±0.82	8.18±0.59	10.47±1.37	<0.001	0.404	0.074
TNF-α (pg/mL)	4.83±0.56	5.83±0.29	5.5±0.44	5.95±0.25	5.07±0.39	5.68±0.36	0.138	0.625	0.289
Leptin (ng/mL)	7.26±0.50	16.55±0.60	16.18±0.59	13.57±0.49^∗	16.52±0.42	16.13±0.55	<0.001	0.005	0.837
		Post-LPS
IL-1β (pg/mL)	100.85±9.2	196.2±15.4	187.1±10.1	149.6±6.7^∗	204.5±10.8	253.1±8.7^†	<0.001	0.016	0.005
TNF-α (pg/mL)	231±8.78	347±10.23	320.75±13.06	288.87±9.49^∗	370.25±14.44	476.79±19.28^†	<0.001	0.004	<0.001
Leptin (ng/mL)	9.18±0.32	18.16±0.83	16.74±0.76	16.27±0.78	16.37±0.8	16.81±0.68	<0.001	0.063	0.238

1Serum cytokines and leptin were measured before LPS challenge (Pre-LPS) and 2 hours following 100 ng LPS challenge (Post-LPS). 2ND: normal diet; HF: high-fat diet; SA: selachyl alcohol; BA: batyl alcohol. 3Values are mean ± SEM. Means with a mark (*) in HF + 20 mg/kg SA or HF + 200 mg/kg SA group differ significantly from the HF diet group. Means with a mark (^†) in HF + 20 mg/kg BA or HF + 200 mg/kg BA group differ significantly from the HF diet group. 4P value (HF) indicates the effect of HF treatment. P value (SA) indicates the effect of different doses of SA supplements on HF fed mice. P value (BA) indicates the effect of different doses of BA supplements on HF fed mice.