Cordycepin Increases Nonrapid Eye Movement Sleep via Adenosine Receptors in Rats

Figure 7

Expression of AR: A₁, A_2A, and A_2B in the rat hypothalamus after cordycepin treatment. AR subtypes in the rat hypothalamus after cordycepin treatment were analyzed by western blotting. The intensity of the immunoreactive bands of 3 to 4 independent experiments was measured by densitometry scanning and normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a standard (bar graph). Results are presented as the percentage immunoreactivity detected in the hypothalamus with respect to the GAPDH protein loading control. Values are expressed as the mean±SEM. ^∗𝑃<0.05,^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.005, compared with that of the control.