Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells

<table>Biochanin A enhanced the osteogenic differentiation in ADSCs. ADSCs were treated for 12 days with a basic medium (negative control) or an osteogenic medium (OM) in the presence of 0.1–1 <i>μ</i>M biochanin A. (a) Osteogenic differentiation was determined by alizarin red S staining of calcium deposits (mineralization) within the cell monolayer. (b) ALP activity was determined using the ALP assay kit according to the manufacturer’s protocol. All results are expressed as the mean <svg height="9.5625" id="M22" style="vertical-align:-0.3003pt" version="1.1" viewbox="0 0 10.1 9.5625" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> compared with the control.</table>

Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Biochanin A Promotes Osteogenic but Inhibits Adipogenic Differentiation: Evidence with Primary Adipose-Derived Stem Cells 