Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells
Figure 2
BDMC attenuated t-BHP-induced alterations of senescence-associated features in WI38 cells. WI38 cells at early passage were treated with 20 μM BDMC for 48 h, followed by t-BHP exposure as described in Materials and Methods to induce premature senescence, and then SA β-gal staining (a) or DAPI staining (b) was performed. (c) Cells were treated with different dose of BDMC (5–40 μM) for 48 h. Then, expressions of p16, p-Rb, and Rb were examined by Western blot assay. (d) The chemical structure of BDMC.
