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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 851940, 11 pages
Research Article

Application of Electron Paramagnetic Resonance Spectroscopy to Comparative Examination of Different Groups of Free Radicals in Thermal Injuries Treated with Propolis and Silver Sulphadiazine

1Department of Community Pharmacy, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
2Department of Biophysics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
3Institute of Computer Science, University of Silesia, 41-200 Sosnowiec, Poland
4Department of Clinical Chemistry and Laboratory Diagnostics, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland
5Center of Experimental Medicine, Medical University of Silesia in Katowice, 40-752 Katowice, Poland

Received 4 April 2013; Accepted 1 May 2013

Academic Editor: Ewelina Szliszka

Copyright © 2013 Pawel Olczyk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Different groups of free radicals expressed in burn wounds treated with propolis and silver sulphadiazine were examined. The thermal effect forms major types of free radicals in a wound because of the breaking of chemical bonds. Free radicals, located in the heated skin, were tested after 21 days of treating by these two substances. The aim of this work was to find the method for determination of types and concentrations of different groups of free radicals in wound after high temperature impact during burning. The effects of the therapy by propolis and silver sulphadiazine on free radicals were studied. Since the chemical methods of free radicals studies are destructive, the usefulness of the electron paramagnetic resonance spectroscopy was tested in this work. The electron paramagnetic resonance spectra measured with the microwave power of 2.2 mW were numerically fitted by theoretical curves of Gaussian and Lorentzian shapes. The experimental electron paramagnetic resonance spectra of tissue samples are best fitted by the sum of one Gauss and two Lorentz lines. An innovatory numerical procedure of spectroscopic skin analysis was presented. It is very useful in the alternative medicine studies.