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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 853976, 11 pages
Research Article

Use of Propolis Hydroalcoholic Extract to Treat Colitis Experimentally Induced in Rats by 2,4,6-Trinitrobenzenesulfonic Acid

1Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maringá, 87020-900 Maringá, PR, Brazil
2Laboratory of Inflammation, Department of Pharmacology and Therapeutics, State University of Maringá, 87020-900 Maringá, PR, Brazil
3Laboratory of Phytotherapy and Apitherapy Development, Department of Pharmacy, State University of Maringá, 87020-900 Maringá, PR, Brazil

Received 9 April 2013; Revised 25 July 2013; Accepted 1 August 2013

Academic Editor: H. Balaji Raghavendran

Copyright © 2013 Cely Cristina Martins Gonçalves et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This study focused on the therapeutic effect of a propolis SLNC 106PI extract on experimental colitis. Wistar adult rats received 0.8 mL rectal dose of one of the following solutions: saline (group S), 20 mg TNBS in 50% ethanol (group TNBS), 20 mg TNBS in 50% ethanol and propolis extract in saline (group TNBS-P), propolis extract in saline (group SP), and 20 mg TNBS in 50% ethanol and 50 mg/kg mesalazine (group TNBS-M). The animals were euthanized 7 or 14 days after the colitis induction. Samples of the distal colon were harvested for the analysis of myeloperoxidase (MPO) enzyme activity and for morphometric analysis in paraffin-embedded histological sections with hematoxylin-eosin or histochemical staining. The animals treated with TNBS exhibited the typical clinical signs of colitis. Increased MPO activity confirmed the presence of inflammation. TNBS induced the development of megacolon, ulceration, transmural inflammatory infiltrate, and thickened bowel walls. Treatment with propolis moderately reduced the inflammatory response, decreased the number of cysts and abscesses, inhibited epithelial proliferation, and increased the number of goblet cells. The anti-inflammatory activity of the propolis SLNC 106 extract was confirmed by the reductions in both the inflammatory infiltrate and the number of cysts and abscesses in the colon mucosa.