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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 856168, 11 pages
Research Article

Repeated-Doses Toxicity Study of the Essential Oil of Hyptis martiusii Benth. (Lamiaceae) in Swiss Mice

1Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50740-521 Recife, PE, Brazil
2Department of Physiology and Pharmacology, Federal University of Pernambuco, 50670-901 Recife, PE, Brazil
3Department of Histology and Embryology, Federal University of Pernambuco, 50670-901 Recife, PE, Brazil
4Department of Physical Education and Sport Sciences, Federal University of Pernambuco, Vitória de Santo Antão, 55608-680 Recife, PE, Brazil
5Department of Biological Chemistry, Regional University of Cariri, 63105-000 Crato, CE, Brazil

Received 16 June 2013; Revised 29 July 2013; Accepted 29 July 2013

Academic Editor: Evan Paul Cherniack

Copyright © 2013 Germana Freire Rocha Caldas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Hyptis martiusii Benth. (Lamiaceae) is found in abundance in Northeastern Brazil where it is used in traditional medicine to treat gastric disorders. Since there are no studies reporting the toxicity and safety profile of this species, we investigated repeated-doses toxicity of the essential oil of Hyptis martiusii (EOHM). Swiss mice of both sexes were orally treated with EOHM (100 and 500 mg/kg) for 30 days, and biochemical, hematological, and morphological parameters were determined. No toxicity signs or deaths were recorded during the treatment with EOHM. The body weight gain was not affected, but there was an occasional variation in water and food consumption among mice of both sexes treated with both doses. The hematological and biochemical profiles did not show significant differences except for a decrease in the MCV and an increase in albumin, but these variations are within the limits described for the species. The microscopic analysis showed changes in liver, kidneys, lungs, and spleen; however, these changes do not have clinical relevance since they varied among the groups, including the control group. The results indicate that the treatment of repeated-doses with the essential oil of Hyptis martiusii showed low toxicity in mice.