Anti-Inflammatory Effects and Mechanisms of Fatsia polycarpa Hayata and Its Constituents
Figure 4
Suppression of the expression of LPS-induced proinflammatory factors by MCE or HOEO. (a), (b), and (c). The analysis by Western blot of the expression of iNOS and COX-2 in RAW 264.7 cells. Cells were treated with MCE (a), brassicasterol (b), or HOEO (c) in the indicated concentrations for 3 h (lanes 3–6 or 3–7), followed by stimulation with LPS for 16 h (lanes 2–6 or 2–7). Band intensities relative to lane 1 in each blot were determined after normalization by the amount of actin and plotted as the respective histogram. (d) and (e), ELISA analysis for the concentration of NO (d) or PGE2 (e) in the culture medium. Cells were treated with 100 μg/mL of MCE (Group 3) or 20 μM of HOEO (Group 4) for 3 h, followed by stimulation with LPS (Groups 2, 3, and 4) for 16 h. Data represent the mean ± SD of three independent experiments. against Group 2. (f), the expression level of IL-1β mRNA analyzed by semiquantitative RT-PCR. Cells were treated as in (d) and (e). The expression of GAPDH was assayed simultaneously as the loading control. Fold represents the relative band intensity against lane 1 after normalization by the amount of GAPDH.
