Figure 2: (a) Untreated control was compared with cells treated at different doses and time. Cells’ morphological changes were observed under phase-contrast microscopy at 40x magnification. Cells shrinkage and rounding were observed clearly. (b) JC-1 dye was used for the analysis of cell mitochondrial membrane potential for flow cytometry as described in Section 2. Cells treated with helichrysetin were found to have lost mitochondrial membrane potential as measured by loss in fluorescence.