Xiao-Ai-Ping, a TCM Injection, Enhances the Antigrowth Effects of Cisplatin on Lewis Lung Cancer Cells through Promoting the Infiltration and Function of CD8+ T Lymphocytes
Figure 4
Xiao-Ai-Ping enhances infiltration and functions of CD8+ T cells in LLC xenografts. PBS (Vehicle, ), 1 mg/kg cisplatin (Cis, ), 20 g crude/kg Xiao-Ai-Ping (Xiao, ), and 1 mg/kg cisplatin plus 20 g crude/kg Xiao-Ai-Ping (Cis+Xiao, ) were daily i.p. injected for 21 days, respectively. Tumor sections were infused in formaldehyde solution for immunofluorescence or shortly frozen in liquid nitrogen for RNA extraction. (a and b) (a) Immunofluorescence of tumor sections. Green, CD8-FITC (Invitrogen); blue, DAPI (4,6-diamidino-2-phenylindole, Sigma). Scale bars, 20 μm. Images were representative of eight samples per group. (b) Quantitation of CD8-FITC (average of eight samples per group). (c and d) Immunohistochemisty stain of tumor sections. (c) Representative images of eight samples per group. Scale bars, 10 μm. (d) Quantitation of GZMB (average of eight samples per group). (e–g) The mRNA levels of gzmb (e), ifn-γ (f), and prf-1 (g) were analyzed by Q-PCR and normalized with rn18s. *, **, ***.
