Melatonin Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances the Antitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells
Figure 2
Effect of melatonin on the expression of 45S pre-rRNA and nucleolar proteins in MDA-MD-231 cells. (a) Effect of melatonin on 45S pre-rRNA. MDA-MB-231 cells were treated with various concentrations of melatonin (0, 3, 6, 12 mM) for 24 h. RNA was isolated and analysed for the expression of 45S pre-rRNA by RT-qPCR. (b) Effect of melatonin on nucleolar proteins by western blotting. Western blotting analysis was performed to detect UBF, fibrillarin, XPO1, IPO7, and -actin. (c) Effect of melatonin on nucleolar proteins by RT-qPCR. RNA was isolated and analysed for the mRNA expression of UBF, fibrillarin, and NCL by RT-qPCR. GAPDH as internal control was used to normalize the expression of data. Data were presented as means ± S.D. , *P < 0.05 versus untreated control.
