Melatonin Suppresses the Expression of 45S Preribosomal RNA and Upstream Binding Factor and Enhances the Antitumor Activity of Puromycin in MDA-MB-231 Breast Cancer Cells
Figure 6
Combined effect of melatonin and puromycin on nucleolar protein and apoptosis related proteins rRNA in MDA-MB-231 cells. (a) Effect of melatonin and/or puromycin on nucleolar proteins by western blotting. (b) Effect of melatonin and/or puromycin on UBF at mRNA level. The mRNA expression of UBF was determined by RT-qPCR. GAPDH was used to normalize the expression of UBF. Data were presented as means S.D. ** versus untreated groups. (c) Effect of melatonin and/or puromycin on procaspase 3, BCL-xL by western blotting.
