Bee Venom Mitigates Cisplatin-Induced Nephrotoxicity by Regulating CD4⁺CD25⁺Foxp3⁺ Regulatory T Cells in Mice

Figure 1

Increased CD4⁺CD25⁺Foxp3⁺ Tregs in vitro and in vivo. BV (1 μg/mL) was treated in splenocytes separated from Foxp3^EGFP mice for 3 days. There was no significant difference in the proportion of dead cells between BV- (20.7%) and PBS-treated (19.3%) groups. This in vitro BV treatment significantly increased the number of CD25⁺Foxp3⁺ T cells among the CD4⁺ T cells (a, b). And Foxp3^EGFP mice were injected with BV (1 mg/kg) or same volume PBS once a day for 5 days. This in vivo BV injection also significantly increased the number of CD25⁺Foxp3⁺ T cells among the CD4⁺ T cells (c). Tregs were analyzed by flow cytometry gated for CD4-positive cells (a). The values shown indicate the mean ± SEM. ^∗∗∗𝑃<0.001 versus control; 𝑛=5 (b), ^∗𝑃<0.05 versus control; 𝑛=3 in vivo (c).

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