Figure 8: Changes in protein expression in response to PC-SPESII treatment. (a, c) MDA-MB-231 cells were treated with or without PC-SPESII for 24 h. Then Western blot analysis was performed using antibodies specific for MMP-2, MMP-9, uPA, uPAR, TIMP-1, TIMP-2, p38MAPK, p-p38MAPK, p-ERK1/2, p-SAPK/JNK, and SAPK/JNK. (b, d) The density ratio of proteins to GAPDH is shown as relative expression. Values are expressed in mean ± SD; three experiments were repeated with similar results. a and b compared to control.