Protective Effects of Huang-Lian-Jie-Du-Tang against Polymicrobial Sepsis Induced by Cecal Ligation and Puncture in Rats
Figure 4
HLJDT augments phagocytic activity of macrophage in septic rats. Rats were orally administrated with 270 mg/kg of HLJDT or vehicle at 2 h prior to CLP or sham surgery. Phagocytotic activities of peritoneal macrophages were evaluated by uptake of FITC-conjugated microsphere at 16 h after CLP or sham surgery. (a) Representative results of flow cytometry analysis of phagocytosis by peritoneal macrophages after incubation with FITC-conjugated microsphere. Region 1(R1) on the dot-plot of side light scatter (SSC) versus forward light scatter (FSC) represented the gate of the macrophages. On the dot-plot of SSC versus green fluorescence (FITC), R2 represented the macrophages uningesting microspheres, and R3, R4, R5, and R6 represented the macrophages that have ingested one, two, three, and four microspheres, respectively. (b) The percentage of phagocytic cells was calculated as the percentage of macrophages that ingested one or more particles. (c) The phagocytic index was calculated as the average number of particles ingested per macrophage (the total number of ingested beads was divided by the number of total macrophages). Data in (b) and (c) are mean ± SEM of 6 rats/group, * versus CLP control, # versus sham control.
