Research Article
Artemisia iwayomogi Extract Attenuates High-Fat Diet-Induced Obesity by Decreasing the Expression of Genes Associated with Adipogenesis in Mice
Table 1
Primer sequences and RT-PCR conditions.
| | Gene description | Primers | Sequences (5′ →3′) | (°C) | Size (bp) |
| | Peroxisome proliferator-activated receptor γ2 (PPARγ2) | F | TTCGGAATCAGCTCTGTGGA | 55 | 148 | | R | CCATTGGGTCAGCTCTTGTG | | | | CCAAT/enhancer binding protein α (C/EBPα) | F | AAGGCCAAGAAGTCGGTGGA | 55 | 189 | | R | CCATAGTGGAAGCCTGATGC | | | | Adipocyte protein 2 (aP2) | F | ACATGAAAGTGGGAGTG | 55 | 128 | | R | AAGTACTCTCTGACCGGATG | | | Cluster of differentiation 36
(CD36) | F | ATGACGTGGCAAAGAACAGC | 55 | 160 | | R | GAAGGCTCAAAGATGCCTCC | | | | Fatty acid synthase (FAS) | F | TTGCCCGAGTCAGAGAACC | 55 | 171 | | R | CGTCCACAATAGCTTCATAGC | | | | Monocyte chemoattractant protein 1 (MCP1) | F | CCAGCAAGATGATCCCAATG | 55 | 450 | | R | CTTCTTGGGGTCAGCACAGA | | | | Interferon α (IFNα) | F | ATGGCTAG(G/A)CTCTGTGCTTTCCT | 60.2 | 500 | | R | GGGCTCTCCAGA(T/C)TTCTGCTCTG | | | | Interferon β (IFNβ) | F | TGGAGCAGCTGAATGGAAAG | 55 | 122 | | R | GAGCATCTCTTGGATGGCAA | | | | Tumor necrosis factor α (TNFα) | F | TGTCTCAGCCTCTTCTCATT | 55 | 156 | | R | AGATGATCTGAGTGTGAGGG | | | | Intereukin 6 (IL-6) | F | TTGCCTTCTTGGGACTGATG | 55 | 162 | | R | CCACGATTTCCCAGAGAACA | | | | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F | AGAACATCATCCCTGCATCC | 60 | 321 | | R | TCCACCACCCTGTTGCTGTA | | |
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