Ursolic-Acid-Enriched Herba Cynomorii Extract Protects against Oxidant Injury in H9c2 Cells and Rat Myocardium by Increasing Mitochondrial ATP Generation Capacity and Enhancing Cellular Glutathione Redox Cycling, Possibly through Mitochondrial Uncoupling
Figure 4
Effect of HCY2 and UA on mitochondrial respiration in H9c2 cells. Cells were preincubated with HCY2 and UA at the indicated concentrations for 4 h and subjected to the measurement of oxygen consumption. Typical oxygen-electrode traces of cellular respiration in H9c2 cells are shown in (a). The effects of HCY2 (b) or UA (c) preincubation on mitochondrial respiration were examined. Data are expressed in percent control with respect to the nonpreincubated control (state 3 respiration rate = 3.34 ± 0.06 nmol O2/min, state 4 respiration rate = 0.78 ± 0.06 nmol O2/min, RCR = 4.37 ± 0.28). Values given are means ± SEM, with . and , when compared with the control group without drug or herbal extract preincubation.
