Ursolic-Acid-Enriched Herba Cynomorii Extract Protects against Oxidant Injury in H9c2 Cells and Rat Myocardium by Increasing Mitochondrial ATP Generation Capacity and Enhancing Cellular Glutathione Redox Cycling, Possibly through Mitochondrial Uncoupling
Figure 8
Effect of HCY2/UA pretreatment on (a) mitochondrial ATP-GC and (b) tissue ATP levels in control perfused and ischemic-reperfused hearts. Biochemical assays of ATP-GC and tissue ATP level were performed as described in Section 2. Data are expressed as the percentage of non-I/R control values. The values of ATP-GC of non I/R control were [AUC2 = 1000 ± 24 (male HCY2), 1000 ± 36 (male UA), 1000 ± 11 (female HCY2), and 1000 ± 21 (female UA)]. The values of tissue ATP level of non-I/R control were 1365 ± 183 (male HCY2), 1024 ± 76 (male UA), 1641 ± 70 (female HCY2), and 1558 ± 240 (female UA) nmol/mg protein. Each bar represents mean ± SEM, with . “*” significantly different from the non-I/R control; “#” significantly different from I/R-challenged control.
