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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 924128, 14 pages
Research Article

Ursolic-Acid-Enriched Herba Cynomorii Extract Protects against Oxidant Injury in H9c2 Cells and Rat Myocardium by Increasing Mitochondrial ATP Generation Capacity and Enhancing Cellular Glutathione Redox Cycling, Possibly through Mitochondrial Uncoupling

Division of Life Science, Hong Kong University of Science & Technology, Clear Water Bay, Hong Kong

Received 2 January 2013; Accepted 11 March 2013

Academic Editor: Tanawan Kummalue

Copyright © 2013 Jihang Chen and Kam Ming Ko. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The ethanol extract of Herba Cynomorii (1.82 kg) was fractionated by silica gel column chromatography, with stepwise elution using a mixture of acetone and petroleum ether (3:7, 1:1 and 7:3; two bed-volumes each), which was followed by absolute ethanol. Four crude fractions, termed, A1, A2, A3 and A4, were obtained. Based on the biological activity (refer to Section 3), A1 and A2 were grouped together and the mixture was again subjected to silica gel column chromatography, with elution by a mixture of acetone and petroleum ether (3:7, v/v) to yield three sub-fractions: HCY1 (57 g), HCY2 (140 g) and HCY3 (16 g).

  1. Supplementary Material