Cucurbitane Triterpenoid from Momordica charantia Induces Apoptosis and Autophagy in Breast Cancer Cells, in Part, through Peroxisome Proliferator-Activated Receptor γ Activation
Figure 2
DMC induces apoptosis in MCF-7 cells. (a) Histograms showing the dose-dependent effect of DMC on Annexin V/PI staining. Cells were treated with DMC at indicated concentrations for 72 h, followed by Annexin V-PI staining. Data are representative of three independent experiments. (b) Dose-dependent effect of DMC on PARP cleavage and caspase-9 activation in MCF-7 cells after 72 h exposure in 5% FBS-supplemented DMEM/F12 medium. (c) Flow cytometry analysis of caspase-3 activity in MCF-7 cells after treatment with DMSO vehicle or the indicated concentrations of DMC for 72 h. 10 μM staurosporine as the positive control. , .
