Research Article

Acetylshikonin, a Novel AChE Inhibitor, Inhibits Apoptosis via Upregulation of Heme Oxygenase-1 Expression in SH-SY5Y Cells

Figure 5

Antiapoptotic activity of acetylshikonin in H2O2 stimulation was related with induction of HO-1 expression in SH-SY5Y cells. (a) Both SH-SY5Y and PC12 cells were treated with desired concentration of acetylshikonin for 12 hours and then stimulated with H2O2 (500 μM) for 1 hours, and HO-1 protein expression was detected by western blot. (b) SH-SY5Y or PC12 cells were pretreated with HO-1 inhibitor (ZnPP, 10 μM) for 2 hours, then incubated with desired concentration of acetylshikonin for 12 hours, further stimulated with H2O2 (500 μM) for 4 hours, and the cell viability (right panel: SH-SY5Y; left panel: PC12) was measured by MTT. Data shown are means ± SEM of results from independent experiments in triplicate. * compared with control cells; # compared with H2O2-stimulated cells; compared with acetylshikonin-treated cells.