Epimedium Flavonoids Counteract the Side Effects of Glucocorticoids on Hypothalamic-Pituitary-Adrenal Axis

Figure 4

Localization and the number of BrdU-incorporated nuclei in the adrenal gland. ((a), (b), (c)) Sections of the adrenal gland from rats of the control group, corticosterone only-treated group, and combined corticosterone and EFs-treated group, respectively, were stained with anti-BrdU antibody and visualized with microscope (magnification ×400). (d) The percentage of BrdU-positive against total cells in zona glomerulosa was calculated. Cells with brown nuclei are considered as BrdU-positive cells. Cap, G, and F denote the capsule, zona glomerulosa, and zona fasciculata, respectively. All sections were counterstained with hematoxylin. ^∗∗𝑃<0.01 versus control group. ^##𝑃<0.01 versus corticosterone-only group.

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