Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 3

Research Article

A Phenylbutenoid Dimer, cis-3-(3′,4′-Dimethoxyphenyl)-4-[(E)-3′′′,4′′′-Dimethoxystyryl] Cyclohex-1-ene, Exhibits Apoptogenic Properties in T-Acute Lymphoblastic Leukemia Cells via Induction of p53-Independent Mitochondrial Signalling Pathway

Figure 3

Confocal micrograph of acridine orange and propidium iodide double-stained CEMss cells after 24, 48, and 72 h treatment with ZC-B11 (IC50). (a) Control, (b) cells exhibit blebbing of the cell membrane and bright green nucleus showing condensation of chromatin (24 h), (c) blebbing was observed with some orange-coloured cells which denotes late apoptosis (48 h), and (d) more blebbing and late apoptosis, orange colour represents the hallmark of late apoptosis while red color represents secondary necrosis or dead cells (72 h). VI: viable cells; BL: blebbing of the cell membrane; CC: chromatin condensation; AB: apoptotic body; LA: late apoptosis; SN: secondary necrosis (400x magnification).