Essential Oil of Pinus koraiensis Exerts Antiobesic and Hypolipidemic Activity via Inhibition of Peroxisome Proliferator-Activated Receptors Gamma Signaling
Figure 1
Effect of EOPK on the differentiation of 3T3-L1 adipocytes. (a) Cytotoxicity of EOPK against 3T3-L1 cells was determined by MTT assay. Cells were treated with various concentrations of EOPK (0, 12.5, 25, or 50 μg/mL) for 24 h. (b, c, and d). Confluent cells were treated with 1 μM dexamethasone, 1 μg/mL insulin, and 0.5 mM IBMX for 2 days, and then the medium was replaced by fresh normal medium only containing 1 μg/mL insulin. On day 8, the differentiated adipocyte cells were exposed to EOPK for 2 days. (b) The differentiated cells were stained with Oil-Red O dye and visualized under inverted microscopy at ×100 magnifications. (c) After dissolving and cellular lipid retained Oil-Red O in isopropanol, adipocyte expression was estimated by measuring OD using microplate reader (Sunrise, TECAN, Mannedorf, Switzerland) at 510 nm. (d) Level of intracellular triglyceride.
