Primo Vascular System Accompanying a Blood Vessel from Tumor Tissue and a Method to Distinguish It from the Blood or the Lymph System
Figure 3
Images of blood vessels with accompanying primo vessels stained with DAPI and Phalloidin. (a) Fluorescent images of primo vessels (arrows) and blood vessels (dotted arrows) stained with Phalloidin. Blood vessels have a smooth muscle structure, whose Phalloidin signal pattern of F-actins had many transversal components to the blood vessel direction. The pattern of F-actins of the two primo vessels (arrows) was only longitudinal and therefore distinct from the patterns of the blood vessels. (b) Fluorescent image of the same sample as that in (a) with DAPI staining of nuclei. Primo vessels have rod-shaped nuclei (arrows). (c) A merged image of (a) and (b). (d) A magnified view of (c). Rod-shaped nuclei (arrows) are clearly seen in the primo vessels which are demonstrably different from the nearby blood vessels.
