Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells
Figure 2
Effects of capsaicin and S-[6]-gingerol on TRPV1 in HuH-7 cells. (a) HuH-7 cells were incubated at 37°C for 6 hours with 10 μM capsaicin or DMSO (vehicle control), respectively. The mRNA levels of TRPV1 were measured using RT-qPCR, normalised to B2M. Results are expressed as mean ± SEM of 3 independent experiments, relative to DMSO controls. ** versus DMSO vehicle control. (b) and (c) HuH-7 cells were cultured in Poly-D-lysine coated 96-well plates and loaded with fluo-4/AM calcium dye. HuH7 cells were preincubated with 40 μM capsazepine (CapZ) for 2 min; then 10 μM capsaicin (b) or 100 μM S-[6]-gingerol (c) was added. DMSO treatment is vehicle control.
