Identification of a Calcium Signalling Pathway of S-[6]-Gingerol in HuH-7 Cells
Figure 3
TRPV1 channels and calcium are associated with S-[6]-gingerol-increased NFκB activity in HuH-7 cells. HuH-7 cells were transfected with an NFκB-luciferase reporter vector. (a) Transfectants were incubated with 100 μM S-[6]-gingerol at 37°C for 7.5, 15, 30, 60, or 120 min. (b) Transfectants were preincubated with 40 μM capsazepine (CapZ) at 37°C for 30 min before incubation with 100 μM S-[6]-gingerol for 7.5, 15, 30, 60, or 120 min. (c) Transfectants were preincubated with 2 mM EGTA at 37°C for 30 min before incubation with 100 μM S-[6]-gingerol for 7.5, 15, 30, 60, or 120 min. Results are expressed as mean ± SEM of 3 independent experiments, relative to DMSO controls. * versus DMSO vehicle control.
