Research Article

Modulation of the Expression of the Proinflammatory IL-8 Gene in Cystic Fibrosis Cells by Extracts Deriving from Olive Mill Waste Water

Figure 5

Effects of all the pure components identified in the olive oil extract on the molecular interactions between NF-κB p50 and -labeled target NF-κB double-stranded oligonucleotide. Derivatives were first incubated with NF-κB, and then the -labeled target NF-κB oligonucleotide was added. NF-κB/DNA complexes were analyzed by polyacrylamide gel electrophoresis. Arrows indicate NF-κB/DNA complexes; asterisks indicate the free -labeled target NF-κB probe.
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