Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway

Figure 3

HG/[6]-GR relieved the oxidative stress induced by DOX in NRCs. (a) LDH release in cell supernatant. (b) Staining of intracellular ROS by DCFH-DA in NRCs (original magnification ×100, bar 100 μm). DCFH-DA is a nonfluorescent analog of fluorescein which will emit fluorescence after being oxidized by intracellular ROS. The bright fluorescence was from the highly fluorescent DCF which indicated the concentration and distribution of ROS. (c) MDA content (d) SOD activity. NRCs were preincubated with or without DOX (5 μM) for 12 h and incubated in the presence or absence of HG/[6]-GR for another 12 h. Data were presented as mean±SD from three independent experiments. *𝑃<0.05, **𝑃<0.01 DOX group versus control group, ^#𝑃<0.05, ^##𝑃<0.01 versus DOX group.

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