Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway
Figure 4
Antiapoptotic effect of HG/[6]-GR in NRCs. NRCs incubated with or without DOX (5 μM) for 12 h, followed by incubation in drug-free medium or HG/[6]-gingerol combination for 12 h. (a) Fluorescence images of DAPI-stained NRCs. The bright parts were the aggregation and fragmentations of chromatin. The condensed and/or irregularly shaped nuclei indicated apoptosis cells (white arrow) (original magnification ×400, bar 100 μm). Quantitative analysis of apoptotic cells by morphological changes and apoptotic nuclear condensation following DAPI staining. (b) Effect of HG/[6]-GR on the expression of caspase-3 activity in NRCs. Cells were similarly treated as indicated. Activation of procaspase-3 was monitored by Western blotting and the relative intensities of protein bands were analyzed by Image J. -Actin was used as an internal control. Experiments were repeated three times and data were shown as meanSD. ** DOX group versus control group, #, ## DOX+HG/[6]-GR group versus DOX group.
