Higenamine Combined with [6]-Gingerol Suppresses Doxorubicin-Triggered Oxidative Stress and Apoptosis in Cardiomyocytes via Upregulation of PI3K/Akt Pathway
Figure 6
Effect of HG/[6]-GR on p-Akt, Akt, and PI3K expression in NRCs. p-Akt, Akt, and PI3K expressions were detected by Western blot analysis. Cells were preincubated with 5 μM DOX for 12 h and then treated with or without indicated concentrations of HG/[6]-GR for another 12 h. PI3K inhibitor LY294002 was added into the culture medium 60 min before cells lysis or harvest. (a) Representative blots for Akt, p-Akt, and PI3K (upper) and quantitative analysis of the ratio of Akt/p-Akt and PI3K compared to -actin (lower) were given. (b) Effect of HG/[6]-GR on cell viability after addition of LY294002 (60 μM). (c) Effect of HG/[6]-GR on p-Akt after addition of LY294002 (60 μM). Data were shown as meanSD from three independent experiments. ** DOX group versus control group, #, ## DOX+HG/[6]-GR group versus DOX group. ▲ versus HG/[6]-GR combination group.
