P90RSK and Nrf2 Activation via MEK1/2-ERK1/2 Pathways Mediated by Notoginsenoside R2 to Prevent 6-Hydroxydopamine-Induced Apoptotic Death in SH-SY5Y Cells
Figure 4
NGR2 could activate P90RSK and Nrf2 pathways in SH-SY5Y cells. The expression of proteins was determined by western blot analysis. HO-1 activities were measured by HO-1 ELISA kits. GSH-PX and GR activities were measured by GSH-PX and GR assay kits, respectively. The SH-SY5Y cells were treated with 20 μM NGR2 for different periods of time (4, 8, 16, and 24 h). (a) NGR2 time dependently increased p-P90RSK and p-BAD expression and nuclear Nrf2 accumulation. (b) NGR2 increased the activities of HO-1, GSH-PX, and GR in a time-dependent manner. The results are expressed as the mean ± SD of three independent experiments. ## indicates a significant difference from the control ().
