Dissection of Mechanisms of a Chinese Medicinal Formula: Danhong Injection Therapy for Myocardial Ischemia/Reperfusion Injury In Vivo and In Vitro

Figure 4

Flow cytometric analysis (a) and TUNEL staining (b) to detect the apoptotic ratio of cardiomyocytes exposed to different treatments followed by SI/R. Images: TUNEL-positive myocytes (stained green, ×40 objective). SI/R, simulated ischemia/reperfusion; A, hydroxysafflor yellow A; B, salvianolic acid B; C, danshensu; DHI, Danhong injection; W, wortmannin; U, U0126. Data are presented as mean ± SD of 3 independent experiments. ^##𝑃<0.01 versus control group; *𝑃<0.05, **𝑃<0.01 versus SI/R group; ^▲𝑃<0.01 versus SI/R + C group; ^△𝑃<0.05 versus SI/R + DHI group.

(a)

(b)