Activation of AMP-Activated Protein Kinase and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells
(a) SW620 cells were treated with CB-PIC (40 g/mL) and/or PD 98059(ERK inhibitor 5 M) for 2 hrs under hypoxia. Western blotting was performed to determine HIF-1, AMPK, pAMPK, pERK, ERK, and -actin expressions. (b) AMPK siRNA decreases the activity of hypoxia-induced apoptosis in SW620 cells under hypoxia. Cells were transiently transfected with AMPK siRNA or control siRNA in the presence or absence of CB-PIC (40 g/mL) under hypoxia. Western blotting was performed to determine the expression of HIF-1, AMPK, pAMPK, pERK, ERK, and -actin.
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