Activation of AMP-Activated Protein Kinase and Extracelluar Signal-Regulated Kinase Mediates CB-PIC-Induced Apoptosis in Hypoxic SW620 Colorectal Cancer Cells
Figure 6
(a) Several concentrations of metformin (0, 0.5, 1, and 5 mM) were treated under hypoxia for 2 hr to determine the expression of AMPK, pAMPK, and -actin. (b) SW 620 cells were treated with CB-PIC (40 g/mL) and/or metformin (5 mM) for 2 hr under hypoxia to investigate of effect of AMPK activator with CB-PIC (40 g/mL). Western blotting was performed to determine HIF-1, AMPK, pAMPK, pERK, ERK, pmTOR, pAKT, AKT, pACC, and -actin expressions.
