Curative Effects of Thiacremonone against Acetaminophen-Induced Acute Hepatic Failure via Inhibition of Proinflammatory Cytokines Production and Infiltration of Cytotoxic Immune Cells and Kupffer Cells
Figure 2
Decreased expression of cytochrome P450 2E1, GSH/GSSG ratio, NO, and lipid peroxidation in liver tissues of thiacremonone pretreated mice. (a) The expression of cytochrome P450 2E1, GSH/GSSG ratio, NO, and lipid peroxidation levels was determined in the total protein extracts of mice liver tissues by western blotting. (b) Immunohistochemical analysis of cytochrome P450 2E1 confirmed the intensities of staining for cytochrome P450 2E1 in the liver tissues of control, APAP-treated, and thiacremonone pretreated mice ((a) and (b)). (c) The hepatic NO in thiacremonone pretreated mice was significantly inhibited compared with that in APAP-treated mice. The expression and intensity of cytochrome P450 2E1 were decreased in the thiacremonone pretreated mice. (d) The hepatic GSH/GSSG ratio in thiacremonone pretreated mice was significantly increased compared with that in APAP-treated mice. (e) Hepatic lipid peroxidation in thiacremonone pretreated mice was significantly inhibited compared with that in APAP-treated mice. Data are mean ± SD; * indicates significant difference from control mice (); # indicates significant difference from APAP-treated mice ().
