Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 2

Research Article

Empetrum nigrum var. japonicum Extract Suppresses Ultraviolet B-Induced Cell Damage via Absorption of Radiation and Inhibition of Oxidative Stress

Figure 2

ENE absorbs UVB light and scavenges UVB-generated intracellular ROS in HaCaT keratinocytes. (a) The UV/visible spectroscopic measurement was conducted in the spectral range of 250–500 nm. Absorbance is reported in absorbance units. Peaks 1, 2, and 3 indicate peak positions at 262 nm, 356 nm, and 367 nm, respectively. (b) Intracellular ROS levels were measured using fluorescence spectrophotometry and (c) flow cytometry. *Significantly different from control cells ( ); **significantly different from UVB-irradiated cells ( ).