Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 4

Research Article

Empetrum nigrum var. japonicum Extract Suppresses Ultraviolet B-Induced Cell Damage via Absorption of Radiation and Inhibition of Oxidative Stress

Figure 4

ENE protects HaCaT keratinocytes against UVB-induced apoptotic body formation and disruption of . (a) Apoptotic bodies (arrows) were observed using fluorescence microscopy and quantified in cells stained with Hoechst 33342. The apoptotic index is defined as the number of apoptotic bodies divided by the total number of intact cells × 100. *Significantly different from control cells ( ); **significantly different from UVB-irradiated cells ( ). (b) Sub-G1 DNA content was measured by flow cytometry. *Significantly different from control cells ( ); **significantly different from UVB-irradiated cells ( ). (c) The was measured by flow cytometry after staining with JC-1. *Significantly different from control cells ( ); **significantly different from UVB-irradiated cells ( ). (d) PI-stained cells were measured using flow cytometry and the dead cell population was shown in the representative dot blot and histogram. *Significantly different from the control ( ); **significantly different from UVB- irradiated cells ( ).
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