Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation on tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats
Table 10
Effects of aqueous rooibos extract, RPO, and/or their combination on glutathione status in the erythrocyte and liver of all experimental rats.
Treatment
Erythrocyte
Liver
GSH (µmol/µg protein)
GSSG (µmol/µg protein)
GSH:GSSG
GSH (µmol/g wet liver)
GSSG (µmol/g wet liver)
GSH:GSSG
Negative control (water)
0.210 ± 0.037
0.121 ± 0.012
1.70 ± 0.20
6.13 ± 0.09
0.46 ± 0.08
18.52 ± 1.57
Positive control (t-BHP)
0.064 ± 0.012*
0.150 ± 0.014
0.41 ± 0.06*
3.84 ± 0.39*
0.56 ± 0.09
8.54 ± 1.63*
RTE
0.190 ± 0.025
0.126 ± 0.011
1.59 ± 0.25
7.82 ± 0.47*
0.23 ± 0.03*
32.54 ± 4.35*
RPO
0.195 ± 0.024
0.109 ± 0.005
1.75 ± 0.16
5.89 ± 0.39
0.23 ± 0.04*
33.05 ± 6.48*
RTE + RPO
0.321 ± 0.027*
0.117 ± 0.003
2.72 ± 0.18*
8.61 ± 0.52*
0.22 ± 0.04*
54.27 ± 10.35*
RTE + t-BHP
0.159 ± 0.026#
0.112 ± 0.005
1.42 ± 0.20#
7.28 ± 0.52*#
0.32 ± 0.04#
24.76 ± 2.96#
RPO + t-BHP
0.182 ± 0.023#
0.109 ± 0.004
1.65 ± 0.19#
5.84 ± 0.29#
0.14 ± 0.01#
41.47 ± 2.58#
RTE + RPO + t-BHP
0.240 ± 0.022#
0.126 ± 0.008
1.89 ± 0.13#
7.72 ± 0.27#
0.37 ± 0.03#
22.92 ± 3.24#
Values are mean ± SEM of 8–10 rats per group. *Significantly different from negative control (). #Significantly different from positive control (). GSH: reduced glutathione, GSSG: oxidised glutathione, RTE: aqueous rooibos extract, RPO: red palm oil, t-BHP: tert-butyl hydroperoxide.
