Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation on tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats
Table 7
Effects of aqueous rooibos, RPO, or their combination on total polyphenol content and antioxidant capacity of plasma and liver of all experimental rats.
Treatment
Plasma
Liver
Total polyphenol content (mg GAE/L)
ORAC (µmol TE/L)
FRAP (µmol AAE/L)
ORAC (µmol TE/g tissue)
FRAP (µmol AAE/g tissue)
Negative control (water)
65.27 ± 2.71
1934.32 ± 101.82
204.85 ± 31.02
15.20 ± 0.39
2.01 ± 0.06
Positive control (t-BHP)
51.11 ± 1.48*
1535.97 ± 50.60*
185.81 ± 11.15
11.51 ± 0.59*
1.99 ± 0.05
RTE
55.38 ± 2.05*
2082.34 ± 88.11
291.75 ± 52.82
14.29 ± 1.23
2.08 ± 0.07
RPO
45.97 ± 1.33*
1284.86 ± 42.14*
210.96 ± 21.18
13.18 ± 0.39*
2.16 ± 0.04
RTE + RPO
56.43 ± 2.60*
1437.41 ± 90.66*
207.84 ± 14.48
14.50 ± 0.75
2.04 ± 0.05
RTE + t-BHP
55.66 ± 3.92*
1721.08 ± 153.85#
243.04 ± 28.98
9.99 ± 1.04*
2.21 ± 0.05
RPO + t-BHP
54.53 ± 2.98*
1296.03 ± 74.88*
207.08 ± 19.47
10.00 ± 0.68*
2.11 ± 0.04
RTE + RPO + t-BHP
54.37 ± 2.43*
1505.31 ± 95.46*
203.91 ± 18.92
10.65 ± 0.85*
2.07 ± 0.04
Values are mean ± SEM of 7–10 rats per group. *Significantly different from negative control group (). #Significantly different from positive control group (). ORAC: oxygen radical absorbance capacity, FRAP: ferric reducing ability of plasma, RTE: aqueous rooibos extract, RPO: red palm oil, t-BHP: tert-butyl hydroperoxide, AAE: ascorbic acid equivalent, GAE: gallic acid equivalent, TE: trolox equivalent.
