Evidence-Based Complementary and Alternative Medicine

Research Article

Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation on tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Table 8

Effects of aqueous rooibos extract, RPO, and/or their combination on antioxidant enzymes activities in erythrocyte and liver of all experimental rats.


Treatment	Erythrocytes				Liver
Treatment	CAT	GR	SOD	GPx	CAT	GR	SOD	GPx

Negative (water)	0.64 ± 0.05	0.56 ± 0.06	5.45 ± 1.71	1.76 ± 0.17	198.90 ± 9.17	17.03 ± 0.50	37.46 ± 4.05	28.41 ± 1.84
Positive (t-BHP)	0.87 ± 0.11**	0.21 ± 0.05*	1.72 ± 0.59*	0.85 ± 0.23*	178.31 ± 5.63**	20.14 ± 1.14*	46.98 ± 3.18	22.10 ± 1.04*
RTE	0.22 ± 0.03*	0.85 ± 0.14	5.96 ± 0.76	1.74 ± 0.14	202.88 ± 5.08	16.16 ± 0.50	38.66 ± 3.04	35.01 ± 1.44*
RPO	0.41 ± 0.02*	0.44 ± 0.05	2.54 ± 0.34	1.62 ± 0.37	197.79 ± 6.23	16.47 ± 0.40	31.14 ± 1.77	29.91 ± 1.19
RTE + RPO	0.20 ± 0.02*	0.42 ± 0.09	7.42 ± 1.56	2.02 ± 0.22	233.26 ± 4.34*	16.43 ± 0.70	34.35 ± 3.49	42.40 ± 4.02*
RTE + t-BHP	0.30 ± 0.02^*#	0.44 ± 0.05^#	4.12 ± 0.89^#	1.67 ± 0.14^#	195.51 ± 6.39	16.25 ± 0.30^#	52.10 ± 3.34	31.45 ± 1.93^#
RPO + t-BHP	0.43 ± 0.17^*#	0.43 ± 0.05^#	1.13 ± 0.25	1.55 ± 0.09^#	186.11 ± 6.01	16.38 ± 0.40^#	38.14 ± 1.86	32.75 ± 2.28^#
RTE + RPO + t-BHP	0.45 ± 0.05^*#	0.40 ± 0.07^#	7.21 ± 1.20^#	2.10 ± 0.41^#	199.37 ± 8.20^#	16.37 ± 0.40^#	36.19 ± 2.39	37.64 ± 3.10^#

Values in columns are mean ± SEM for 7–10 rats per group. *Significantly different from negative control (). **Marginally different from negative control (). ^#Significantly different from positive control (). CAT: catalase, µmol H₂O₂ consumed/min/µg protein in the erythrocyte or µmol H₂O₂ consumed/min/mg protein in the liver, GR: glutathione reductase, µmol NADPH oxidized/min/µg protein in the erythrocyte or µmol NADPH oxidized/min/mg protein in the liver, SOD: superoxide dismutase, U/µg protein in erythrocyte or U/mg protein in the liver, GPx: glutathione peroxidase, nmol NADPH oxidized/min/µg protein in the erythrocyte or nmol NADPH oxidized/min/mg protein in the liver, RTE: aqueous rooibos extract, RPO: red palm oil, t-BHP: tert-butyl hydroperoxide.