Antidiabetic Effect of Methanolic Extract from Berberis julianae Schneid. via Activation of AMP-Activated Protein Kinase in Type 2 Diabetic Mice
Figure 1
BJSME stimulated IRAP trafficking in L6 cell. (a) L6 cells were infected with pIRAP-mOrange in order to detect externalized IRAP by confocal microscopy. Confocal images in L6 cells incubated in the absence (basal) or presence of BJSME for 25 minutes. Scale bar: 40 μm. (b) Time course of the change in fluorescence at PM induced by insulin ( cells) and BJSME ( cells) in IRAP-mOrange transfected L6 cells. Data represent three independent experiments. (c) M ± SEM of the change in fluorescence from (b). Data represent the fold increase in fluorescence induced by insulin and BJSME between 0 and 25 minutes. IRAP-mOrange surface labeling in experiments was measured in L6 cells under insulin and BJSME-treated conditions. Data represent M ± SEM of values from three separate experiments. compared to basal. (d) Confocal images in L6 cells incubated in the absence (basal) or presence of Compound C and BJSME for 25 minutes. Scale bar: 40 μm. (e) Time course of the change in fluorescence induced by BJSME ( cells) and Compound C with BJSME ( cells) in IRAP-mOrange transfected L6 cells. Data represent three independent experiments. (f) Mean ± SEM of arbitrary units of the change in fluorescence from (e). Data represent the fold increase in fluorescence induced by Compound C and BJSME from 0 to 25 minutes. The fluorescence of L6 cells treated by BJSME compared with BJSME in Compound C has significant difference ().
